Nitrocefin Applications:
Nitrocefin is widely used in microbiology laboratories as a diagnostic tool to detect the presence of β-lactamase in clinically isolated bacteria, which can confer resistance to β-lactam antibiotics such as penicillins and cephalosporins.
In the development of novel antibiotics, it is used for competitive inhibition studies of β-lactamase, aiding in the screening of effective inhibitors.
Research on antibiotic resistance: It is employed to study the resistance mechanisms of bacteria to β-lactam antibiotics, providing support for the development of new antibiotics.
Chemical Properties
| Catalogue number: |
TA1016 |
| TA1016 |
Nitrocefin |
| Synonyms: |
(6R,7R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid; [6R-[3(E),6α,7β]]-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid; |
| CAS Number: |
41906-86-9 |
| Possible CAS #: |
NA |
| Molecular form.: |
C21 H16 N4 O8 S2 |
| Appearance: |
Orange to Red Solid |
| Melting Point: |
>99°C (dec.) |
| Storage: |
Hygroscopic, -20°C Freezer, Under inert atmosphere |
| Solubility: |
DMSO (Slightly), Methanol (Slightly) |
Nitrocefin Usage Method
-1.1 Glass slide surface analysis method
a) Take a drop of Cefnitrothiophene working solution (1mg/ml) onto a clean glass slide surface;
b) Use a sterile inoculum ring to select a solid culture clone and mix it with liquid Chemicalbook drops evenly;
c) If red color can be observed within 20-30 minutes, it indicates that β- Lactam enzyme activity. If necessary, add more drops of Cefnitrothiophene working solution to prevent dryness
–1.2 Direct contact analysis method
a) Directly suck a drop of Cefnitrothiophene working solution (1mg/ml) onto the surface of the isolated colony;
b) If red color can be observed within 20-30 minutes, it indicates that β- Lactam enzyme activity.
–1.3 Broth culture medium analysis method
a) Take 1ml of broth culture medium and add 3-5 drops of Cefnitrothiophene working solution (1mg/ml);
b) If red color can be observed within 20-30 minutes, it indicates that β- Lactam enzyme activity.
–1.4 Cell lysate analysis method
a) Ultrasound lysis of 1ml cell suspension;
b) Add 3-5 drops of Cefnitrothiophene working solution (1mg/ml) to the lysis suspension; c) If red color can be observed within 20-30 minutes, it indicates that β- Lactam enzyme activity.
–1.5 Filter paper analysis method
a) Saturate a small piece of filter paper (~3x3cm) to absorb Cefnitrothiophene working solution (1mg/ml), and then place it in a clean culture dish;
b) Jump a separated clone and apply it to the impregnated filter paper;
c) If red color can be observed within 20-30 minutes, it indicates that β- Lactam enzyme activity.
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