{"id":260,"date":"2024-08-22T16:39:09","date_gmt":"2024-08-22T08:39:09","guid":{"rendered":"https:\/\/www.csyuteng.com\/?p=260"},"modified":"2025-12-26T10:41:24","modified_gmt":"2025-12-26T02:41:24","slug":"nitrocefin","status":"publish","type":"post","link":"https:\/\/www.csyuteng.com\/fr\/nitrocefin.html","title":{"rendered":"Nitroc\u00e9fine"},"content":{"rendered":"

Applications de la nitroc\u00e9fine :<\/strong><\/p>\n

La nitroc\u00e9fine est largement utilis\u00e9e dans les laboratoires de microbiologie comme outil de diagnostic pour d\u00e9tecter la pr\u00e9sence de \u03b2-lactamase dans les bact\u00e9ries isol\u00e9es cliniquement, qui peut conf\u00e9rer une r\u00e9sistance aux antibiotiques \u03b2-lactamines tels que les p\u00e9nicillines et les c\u00e9phalosporines.
\nDans le cadre du d\u00e9veloppement de nouveaux antibiotiques, il est utilis\u00e9 pour des \u00e9tudes d'inhibition comp\u00e9titive de la \u03b2-lactamase, ce qui facilite le criblage d'inhibiteurs efficaces.
\nRecherche sur la r\u00e9sistance aux antibiotiques : Il est utilis\u00e9 pour \u00e9tudier les m\u00e9canismes de r\u00e9sistance des bact\u00e9ries aux \u03b2-lactamines, ce qui permet de soutenir le d\u00e9veloppement de nouveaux antibiotiques.<\/p>\n

 <\/p>\n

Propri\u00e9t\u00e9s chimiques<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Num\u00e9ro de catalogue :<\/td>\nTA1016<\/td>\n<\/tr>\n
TA1016<\/td>\nNitroc\u00e9fine<\/td>\n<\/tr>\n
Synonymes :<\/td>\n(6R,7R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid; [6R-[3(E),6\u03b1,7\u03b2]]-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid;<\/td>\n<\/tr>\n
Num\u00e9ro CAS :<\/td>\n41906-86-9<\/td>\n<\/tr>\n
Possible CAS # :<\/td>\nNA<\/td>\n<\/tr>\n
Forme mol\u00e9culaire :<\/td>\nC21<\/sub>\u00a0H16<\/sub>\u00a0N4<\/sub>\u00a0O8<\/sub>\u00a0S2<\/sub><\/td>\n<\/tr>\n
Apparence :<\/td>\nOrange \u00e0 rouge solide<\/td>\n<\/tr>\n
Point de fusion :<\/td>\n>99\u00b0C (d\u00e9c.)<\/td>\n<\/tr>\n
Stockage :<\/td>\nHygroscopique, -20\u00b0C Cong\u00e9lateur, Sous atmosph\u00e8re inerte<\/td>\n<\/tr>\n
Solubilit\u00e9 :<\/td>\nDMSO (l\u00e9g\u00e8rement), m\u00e9thanol (l\u00e9g\u00e8rement)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

M\u00e9thode d'utilisation de la nitroc\u00e9fine<\/strong><\/p>\n

-1.1 M\u00e9thode d'analyse de la surface de la lame de verre<\/p>\n

a) Pr\u00e9lever une goutte de solution de travail de Cefnitrothioph\u00e8ne (1mg\/ml) sur une lame de verre propre ;<\/p>\n

b) Utiliser un anneau d'inoculum st\u00e9rile pour s\u00e9lectionner un clone de culture solide et le m\u00e9langer uniform\u00e9ment avec les gouttes liquides de Chemicalbook ;<\/p>\n

c) Si une couleur rouge peut \u00eatre observ\u00e9e dans les 20-30 minutes, cela indique l'activit\u00e9 de l'enzyme \u03b2- Lactam. Si n\u00e9cessaire, ajouter des gouttes suppl\u00e9mentaires de solution de travail de Cefnitrothioph\u00e8ne pour \u00e9viter le dess\u00e8chement.<\/p>\n

-1.2 M\u00e9thode d'analyse par contact direct<\/p>\n

a) Aspirer directement une goutte de solution de travail de Cefnitrothioph\u00e8ne (1mg\/ml) sur la surface de la colonie isol\u00e9e ;<\/p>\n

b) Si une couleur rouge peut \u00eatre observ\u00e9e dans les 20-30 minutes, cela indique une activit\u00e9 enzymatique \u03b2- Lactam.<\/p>\n

-1.3 M\u00e9thode d'analyse du milieu de culture en bouillon<\/p>\n

a) Pr\u00e9lever 1 ml de milieu de culture en bouillon et ajouter 3 \u00e0 5 gouttes de solution de travail de Cefnitrothioph\u00e8ne (1mg\/ml) ;<\/p>\n

b) Si une couleur rouge peut \u00eatre observ\u00e9e dans les 20-30 minutes, cela indique une activit\u00e9 enzymatique \u03b2- Lactam.<\/p>\n

-1.4 M\u00e9thode d'analyse du lysat cellulaire<\/p>\n

a) Lyse par ultrasons d'une suspension cellulaire de 1 ml ;<\/p>\n

b) Ajouter 3-5 gouttes de solution de travail de Cefnitrothioph\u00e8ne (1mg\/ml) \u00e0 la suspension de lyse ; c) Si une couleur rouge peut \u00eatre observ\u00e9e dans les 20-30 minutes, cela indique l'activit\u00e9 de l'enzyme \u03b2- Lactam.<\/p>\n

-1.5 M\u00e9thode d'analyse sur papier filtre<\/p>\n

a) Saturer un petit morceau de papier filtre (~3x3cm) pour absorber la solution de travail de Cefnitrothiophene (1mg\/ml), puis le placer dans une bo\u00eete de culture propre ;<\/p>\n

b) Pr\u00e9lever un clone s\u00e9par\u00e9 et l'appliquer sur le papier filtre impr\u00e9gn\u00e9 ;<\/p>\n

c) Si une couleur rouge peut \u00eatre observ\u00e9e dans les 20-30 minutes, cela indique une activit\u00e9 enzymatique \u03b2- Lactam.<\/p>","protected":false},"excerpt":{"rendered":"

Nitrocefin Applications: Nitrocefin is widely used in microbiology laboratories as a diagnostic tool to detect the presence of \u03b2-lactamase in clinically isolated bacteria, which can confer resistance to \u03b2-lactam antibiotics such as penicillins and cephalosporins. In the development of novel antibiotics, it is used for competitive inhibition studies of \u03b2-lactamase, aiding in the screening of […]<\/p>","protected":false},"author":1,"featured_media":261,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-260","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-nitrocefin"],"yoast_head":"\nNitrocefin CAS: 41906-86-9 Manufacturer - Changsha Yuteng New Materials Co., Ltd.<\/title>\n<meta name=\"description\" content=\"Changsha Yuteng New Materials Co., Ltd. is a comprehensive enterprise integrating R&D, production and operation. 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