{"id":260,"date":"2024-08-22T16:39:09","date_gmt":"2024-08-22T08:39:09","guid":{"rendered":"https:\/\/www.csyuteng.com\/?p=260"},"modified":"2025-12-26T10:41:24","modified_gmt":"2025-12-26T02:41:24","slug":"nitrocefin","status":"publish","type":"post","link":"https:\/\/www.csyuteng.com\/es\/nitrocefin.html","title":{"rendered":"Nitrocefina"},"content":{"rendered":"

Aplicaciones de la nitrocefina:<\/strong><\/p>\n

La nitrocefina se utiliza ampliamente en los laboratorios de microbiolog\u00eda como herramienta de diagn\u00f3stico para detectar la presencia de \u03b2-lactamasa en bacterias aisladas cl\u00ednicamente, lo que puede conferir resistencia a antibi\u00f3ticos \u03b2-lact\u00e1micos como penicilinas y cefalosporinas.
\nEn el desarrollo de nuevos antibi\u00f3ticos, se utiliza para estudios de inhibici\u00f3n competitiva de \u03b2-lactamasa, ayudando en la selecci\u00f3n de inhibidores eficaces.
\nInvestigaci\u00f3n sobre la resistencia a los antibi\u00f3ticos: Se emplea para estudiar los mecanismos de resistencia de las bacterias a los antibi\u00f3ticos \u03b2-lact\u00e1micos, proporcionando apoyo para el desarrollo de nuevos antibi\u00f3ticos.<\/p>\n

 <\/p>\n

Propiedades qu\u00edmicas<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
N\u00famero de cat\u00e1logo:<\/td>\nTA1016<\/td>\n<\/tr>\n
TA1016<\/td>\nNitrocefina<\/td>\n<\/tr>\n
Sin\u00f3nimos:<\/td>\n(6R,7R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid; [6R-[3(E),6\u03b1,7\u03b2]]-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid;<\/td>\n<\/tr>\n
N\u00famero CAS:<\/td>\n41906-86-9<\/td>\n<\/tr>\n
Posible CAS #:<\/td>\nNA<\/td>\n<\/tr>\n
Forma molecular.:<\/td>\nC21<\/sub>\u00a0H16<\/sub>\u00a0N4<\/sub>\u00a0O8<\/sub>\u00a0S2<\/sub><\/td>\n<\/tr>\n
Apariencia:<\/td>\nNaranja a rojo s\u00f3lido<\/td>\n<\/tr>\n
Punto de fusi\u00f3n:<\/td>\n>99\u00b0C (dec.)<\/td>\n<\/tr>\n
Almacenamiento:<\/td>\nHigrosc\u00f3pico, -20\u00b0C Congelador, Bajo atm\u00f3sfera inerte<\/td>\n<\/tr>\n
Solubilidad:<\/td>\nDMSO (ligeramente), Metanol (ligeramente)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

M\u00e9todo de utilizaci\u00f3n de la nitrocefina<\/strong><\/p>\n

-1.1 M\u00e9todo de an\u00e1lisis de la superficie del portaobjetos<\/p>\n

a) Tomar una gota de soluci\u00f3n de trabajo de Cefnitrotiofeno (1mg\/ml) sobre una superficie limpia de portaobjetos de vidrio;<\/p>\n

b) Utilice un anillo de in\u00f3culo est\u00e9ril para seleccionar un clon de cultivo s\u00f3lido y m\u00e9zclelo con gotas de Chemicalbook l\u00edquido de manera uniforme;<\/p>\n

c) Si se observa color rojo en 20-30 minutos, indica que hay actividad enzim\u00e1tica \u03b2- Lactam. Si es necesario, a\u00f1adir m\u00e1s gotas de soluci\u00f3n de trabajo de Cefnitrotiofeno para evitar la sequedad.<\/p>\n

-1.2 M\u00e9todo de an\u00e1lisis por contacto directo<\/p>\n

a) Aspirar directamente una gota de soluci\u00f3n de trabajo de Cefnitrotiofeno (1mg\/ml) sobre la superficie de la colonia aislada;<\/p>\n

b) Si se observa color rojo en 20-30 minutos, indica que hay actividad enzim\u00e1tica \u03b2- Lactam.<\/p>\n

-1.3 M\u00e9todo de an\u00e1lisis del medio de cultivo en caldo<\/p>\n

a) Tomar 1 ml de medio de cultivo de caldo y a\u00f1adir 3-5 gotas de soluci\u00f3n de trabajo de Cefnitrotiofeno (1mg\/ml);<\/p>\n

b) Si se observa color rojo en 20-30 minutos, indica que hay actividad enzim\u00e1tica \u03b2- Lactam.<\/p>\n

-1.4 M\u00e9todo de an\u00e1lisis del lisado celular<\/p>\n

a) Lisis por ultrasonidos de 1 ml de suspensi\u00f3n celular;<\/p>\n

b) A\u00f1adir 3-5 gotas de soluci\u00f3n de trabajo de Cefnitrotiofeno (1mg\/ml) a la suspensi\u00f3n de lisis; c) Si se observa color rojo en 20-30 minutos, indica que hay actividad de la enzima \u03b2- Lactam.<\/p>\n

-1.5 M\u00e9todo de an\u00e1lisis con papel de filtro<\/p>\n

a) Saturar un peque\u00f1o trozo de papel de filtro (~3x3cm) para absorber la soluci\u00f3n de trabajo de Cefnitrotiofeno (1mg\/ml), y luego colocarlo en una placa de cultivo limpia;<\/p>\n

b) Saltar un clon separado y aplicarlo al papel de filtro impregnado;<\/p>\n

c) Si se observa color rojo en 20-30 minutos, indica que hay actividad de la enzima \u03b2- Lactam.<\/p>","protected":false},"excerpt":{"rendered":"

Aplicaciones de la nitrocefina: La nitrocefina se utiliza ampliamente en los laboratorios de microbiolog\u00eda como herramienta de diagn\u00f3stico para detectar la presencia de \u03b2-lactamasa en bacterias aisladas cl\u00ednicamente, que puede conferir resistencia a antibi\u00f3ticos \u03b2-lact\u00e1micos como penicilinas y cefalosporinas. En el desarrollo de nuevos antibi\u00f3ticos, se utiliza para estudios de inhibici\u00f3n competitiva de \u03b2-lactamasa, ayudando en el cribado de [...]<\/p>","protected":false},"author":1,"featured_media":261,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-260","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-nitrocefin"],"yoast_head":"\nNitrocefin CAS: 41906-86-9 Manufacturer - Changsha Yuteng New Materials Co., Ltd.<\/title>\n<meta name=\"description\" content=\"Changsha Yuteng New Materials Co., Ltd. is a comprehensive enterprise integrating R&D, production and operation. 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