{"id":260,"date":"2024-08-22T16:39:09","date_gmt":"2024-08-22T08:39:09","guid":{"rendered":"https:\/\/www.csyuteng.com\/?p=260"},"modified":"2025-12-26T10:41:24","modified_gmt":"2025-12-26T02:41:24","slug":"nitrocefin","status":"publish","type":"post","link":"https:\/\/www.csyuteng.com\/de\/nitrocefin.html","title":{"rendered":"Nitrocefin"},"content":{"rendered":"

Nitrocefin Anwendungen:<\/strong><\/p>\n

Nitrocefin wird in mikrobiologischen Labors h\u00e4ufig als Diagnoseinstrument verwendet, um das Vorhandensein von \u03b2-Lactamase in klinisch isolierten Bakterien nachzuweisen, die eine Resistenz gegen \u03b2-Lactam-Antibiotika wie Penicilline und Cephalosporine verleihen k\u00f6nnen.
\nBei der Entwicklung neuartiger Antibiotika wird es f\u00fcr Studien zur kompetitiven Hemmung von \u03b2-Lactamase verwendet, was beim Screening wirksamer Hemmstoffe hilfreich ist.
\nForschung zur Antibiotikaresistenz: Es wird eingesetzt, um die Resistenzmechanismen von Bakterien gegen \u03b2-Laktam-Antibiotika zu untersuchen und so die Entwicklung neuer Antibiotika zu unterst\u00fctzen.<\/p>\n

 <\/p>\n

Chemische Eigenschaften<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Katalognummer:<\/td>\nTA1016<\/td>\n<\/tr>\n
TA1016<\/td>\nNitrocefin<\/td>\n<\/tr>\n
Synonyme:<\/td>\n(6R,7R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid; [6R-[3(E),6\u03b1,7\u03b2]]-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid;<\/td>\n<\/tr>\n
CAS-Nummer:<\/td>\n41906-86-9<\/td>\n<\/tr>\n
M\u00f6glicherweise CAS #:<\/td>\nNA<\/td>\n<\/tr>\n
Molekulare Form:<\/td>\nC21<\/sub>\u00a0H16<\/sub>\u00a0N4<\/sub>\u00a0O8<\/sub>\u00a0S2<\/sub><\/td>\n<\/tr>\n
Erscheinungsbild:<\/td>\nOrange bis Rot fest<\/td>\n<\/tr>\n
Schmelzpunkt:<\/td>\n>99\u00b0C (dez.)<\/td>\n<\/tr>\n
Lagerung:<\/td>\nHygroskopisch, -20\u00b0C Gefrierschrank, unter inerter Atmosph\u00e4re<\/td>\n<\/tr>\n
L\u00f6slichkeit:<\/td>\nDMSO (geringf\u00fcgig), Methanol (geringf\u00fcgig)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Nitrocefin Verwendungsmethode<\/strong><\/p>\n

-1.1 Methode zur Analyse der Glasobjekttr\u00e4geroberfl\u00e4che<\/p>\n

a) Nehmen Sie einen Tropfen der Cefnitrothiophen-Arbeitsl\u00f6sung (1 mg\/ml) auf einen sauberen Objekttr\u00e4ger aus Glas;<\/p>\n

b) Verwenden Sie einen sterilen Inokulum-Ring, um einen festen Kulturklon auszuw\u00e4hlen, und mischen Sie ihn gleichm\u00e4\u00dfig mit fl\u00fcssigen Chemicalbook-Tropfen;<\/p>\n

c) Wenn innerhalb von 20-30 Minuten eine rote F\u00e4rbung zu beobachten ist, deutet dies auf eine Aktivit\u00e4t des \u03b2-Lactam-Enzyms hin. Falls erforderlich, weitere Tropfen Cefnitrothiophen-Arbeitsl\u00f6sung zugeben, um ein Austrocknen zu verhindern.<\/p>\n

-1.2 Methode der direkten Kontaktanalyse<\/p>\n

a) Einen Tropfen Cefnitrothiophen-Arbeitsl\u00f6sung (1mg\/ml) direkt auf die Oberfl\u00e4che der isolierten Kolonie saugen;<\/p>\n

b) Wenn innerhalb von 20-30 Minuten eine rote Farbe beobachtet werden kann, deutet dies auf eine \u03b2-Lactam-Enzymaktivit\u00e4t hin.<\/p>\n

-1.3 Methode zur Analyse des N\u00e4hrbodens<\/p>\n

a) Nehmen Sie 1 ml Br\u00fchkulturmedium und geben Sie 3-5 Tropfen Cefnitrothiophen-Arbeitsl\u00f6sung (1 mg\/ml) hinzu;<\/p>\n

b) Wenn innerhalb von 20-30 Minuten eine rote Farbe beobachtet werden kann, deutet dies auf eine \u03b2-Lactam-Enzymaktivit\u00e4t hin.<\/p>\n

-1.4 Methode zur Analyse von Zelllysaten<\/p>\n

a) Ultraschall-Lyse von 1 ml Zellsuspension;<\/p>\n

b) 3-5 Tropfen Cefnitrothiophen-Arbeitsl\u00f6sung (1mg\/ml) in die Lysesuspension geben; c) Wenn innerhalb von 20-30 Minuten eine rote Farbe zu beobachten ist, deutet dies auf eine \u03b2-Lactam-Enzymaktivit\u00e4t hin.<\/p>\n

-1.5 Filterpapier-Analyseverfahren<\/p>\n

a) Tr\u00e4nken Sie ein kleines St\u00fcck Filterpapier (~3x3cm), um die Cefnitrothiophen-Arbeitsl\u00f6sung (1mg\/ml) aufzusaugen, und legen Sie es dann in eine saubere Kulturschale;<\/p>\n

b) Springen Sie einen abgetrennten Klon und tragen Sie ihn auf das impr\u00e4gnierte Filterpapier auf;<\/p>\n

c) Wenn innerhalb von 20-30 Minuten eine rote Farbe beobachtet werden kann, deutet dies auf eine \u03b2-Lactam-Enzymaktivit\u00e4t hin.<\/p>","protected":false},"excerpt":{"rendered":"

Nitrocefin Applications: Nitrocefin is widely used in microbiology laboratories as a diagnostic tool to detect the presence of \u03b2-lactamase in clinically isolated bacteria, which can confer resistance to \u03b2-lactam antibiotics such as penicillins and cephalosporins. In the development of novel antibiotics, it is used for competitive inhibition studies of \u03b2-lactamase, aiding in the screening of […]<\/p>","protected":false},"author":1,"featured_media":261,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-260","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-nitrocefin"],"yoast_head":"\nNitrocefin CAS: 41906-86-9 Manufacturer - Changsha Yuteng New Materials Co., Ltd.<\/title>\n<meta name=\"description\" content=\"Changsha Yuteng New Materials Co., Ltd. is a comprehensive enterprise integrating R&D, production and operation. The company is dominated by R&D and is committed to the R&D, production and sales of pharmaceutical intermediates, pesticide intermediates and cosmetic raw materials.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.csyuteng.com\/de\/nitrocefin.html\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nitrocefin CAS: 41906-86-9 Manufacturer - Changsha Yuteng New Materials Co., Ltd.\" \/>\n<meta property=\"og:description\" content=\"Changsha Yuteng New Materials Co., Ltd. is a comprehensive enterprise integrating R&D, production and operation. 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